重组Anti-LI Cadherin抗体[EPR3996] (ab109190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3996] to LI Cadherin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-LI Cadherin抗体[EPR3996]
参阅全部 LI Cadherin 一抗 -
描述
兔单克隆抗体[EPR3996] to LI Cadherin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解离常数(KD)
KD = 2.90 x 10 -12 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3996 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport. -
组织特异性
Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin. -
序列相似性
Contains 7 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 1015 Human
- Omim: 603017 Human
- SwissProt: Q12864 Human
- Unigene: 591853 Human
-
别名
- BILL-cadherin antibody
- CAD17_HUMAN antibody
- Cadherin 16 antibody
see all
图片
-
Lanes 1-3 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (Purified)
Lanes 4-5 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution
Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Human small intestine lysate
Lane 3 : Human colon lysate
Lane 4 : Human spleen lysate
Lane 5 : Human kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution
Lane 1 : Human small intestine cell lysate
Lane 2 : Human colon cancer cell lysate
Lane 3 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 92 kDa -
Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
ab109190 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab109190 尚未被引用在任何文献中。