Anti-LANCL2抗体(ab88860)
Key features and details
- Mouse polyclonal to LANCL2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LANCL2抗体
参阅全部 LANCL2 一抗 -
描述
小鼠多克隆抗体to LANCL2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human LANCL2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: A431 cell lysate; LANCL2 transfected 293T cell lysate ICC/IF: HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes. -
组织特异性
Expressed in brain and testis. -
序列相似性
Belongs to the LanC-like protein family. -
翻译后修饰
Myristoylated. Essential for membrane association. -
细胞定位
Nucleus. Cytoplasm. Cell membrane. Localizes to the juxta-nuclear vesicles. Associates with the cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-cyclodextrin causes partial dissociation from the cell membrane in vitro and an enhanced cell detachment from the matrix in vivo. Membrane-association is important for the increased cellular sensitivity to an anticancer drug. - Information by UniProt
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数据库链接
- Entrez Gene: 55915 Human
- SwissProt: Q9NS86 Human
- Unigene: 595384 Human
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别名
- G protein coupled receptor 69B antibody
- GPR69B antibody
- LanC (bacterial lantibiotic synthetase component C) like 2 antibody
see all
图片
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Anti-LANCL2 antibody (ab88860) at 1 µg/ml + A431 cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: ~48 kDa why is the actual band size different from the predicted? -
All lanes : Anti-LANCL2 antibody (ab88860) at 1 µg/ml
Lane 1 : LANCL2 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: ~45 kDa why is the actual band size different from the predicted? -
ab88860, at 10 µg/ml, staining LANCL2 in paraformaldehyde fixed, permeabilized HeLa cell by Immunofluorescence.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab88860 被引用在 1 文献中.
- Lou Y et al. Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis. Cell Death Dis 12:170 (2021). PubMed: 33568630