Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS containing 0.1% Tween20 and 5% milk
Dilution
1/300
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: HRP conjugated Rabbit-anti Rat
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 105 kDa Non-specific: 80-90, 140, 160, kDa
Additional data
Additional Notes
[Membrane used] : 0.2um_Nitrocellulose
[Lysis buffer used] : Commercial RIPA buffer
(150mM Sodium chloride, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 50mM Tri-HCl, pH=7.5, and 2mM DETA, sterile solution)
[Protease inhibitors used] : Halt Protease and Phosphatase Inhibitor Cocktail (Cat No. 78440)
Abcam response
Abcam wishes to thank the reviewer for this submission. Please note, Ratis not a validated species for ab13524
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Insu Kwon
Verified customer
提交于 Dec 10 2015