Anti-L-Alanine抗体(ab9436)
Key features and details
- Rabbit polyclonal to L-Alanine
- Suitable for: IHC-FoFr, ICC, IHC-Fr, Immunomicroscopy
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-L-Alanine抗体 -
描述
兔多克隆抗体to L-Alanine -
宿主
Rabbit -
特异性
The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against alanine in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against D/L-aspartate, D/L-arginine, agmatine, guanidine, gamma-aminobutyrate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Significant cross-reactivity (1:10) was detected against beta-alanine. -
经测试应用
适用于: IHC-FoFr, ICC, IHC-Fr, Immunomicroscopymore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to L-Alanine (Glutaraldehyde).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
Concentration information loading...
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纯度
IgG fraction -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-FoFr | ||
ICC | ||
IHC-Fr | ||
Immunomicroscopy |
说明 |
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Recommended user dilution: 1/100
True Dilution at User Dilution: 1/8000
High-performance thin section immunostaining using silver-intensified immunogold or fluorescence detection.
Enhanced detection is possible using streptavidin detection.
EM applications with 10-40 nm gold GAR IgGs or gold streptavidin.
OPTIMAL FIXATION: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten).
MINIMAL GLUTARALDEHYDE: 0.1% using HPI
The hapten is osmium tolerant (deosmication required), therefore the antisera can be used on conventional post-embedding electron microscope immunostaining.
All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.
Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.
数据表及文件
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Datasheet download
文献 (1)
ab9436 被引用在 1 文献中.
- Sun D et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC ; Rat . PubMed: 17729258