Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Cy3-AffiniPure F(ab')2 Fragment Goat Anti-Rabbit I
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
very weak staining
Discount code: 53377
Discount code: 53377
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in immunocytochemistry using mouse sample. This antibody has not been tested with this species before, and it is possible that some optimization may be required to increase the signal or the product is not suitable for use with mouse sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 05 2011