Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS - Tween
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Abcam response
We are pleased to see this antibody has provided some results in mouse tissue, thank you for your valuable feedback. However, as we have previously received feedback to indicate this antibody does not work so well in mouse (as noted on the main datasheet page). This may also be why the results in mouse have been given a three star rating for this Abreview. We do not guarantee this antibody for use in mouse for this reason.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 20 2009