Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Permeabilization
Yes - triton
Specification
prostate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti mouse
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I purchased this antibody to test IF. It was not one of the recommended application. So, I was offered 'AbTrial program'. I would like to report that this antibody does not work for IF on primary prostate epithelial cells and on prostate cell lines. Thanks for offering AbTrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 28 2014