Application
Western blot
Sample
Human Cell lysate - whole cell (B lymphocytes)
Loading amount
15 µg
Specification
B lymphocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 20 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse AP (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 11 kDa
Positive control
Human B lymphocyte lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 06 2008