重组HRP Anti-Kappa light chain抗体[EPR5367-8] (ab202549)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5367-8] to Kappa light chain
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-Kappa light chain抗体[EPR5367-8]
参阅全部 Kappa light chain 一抗 -
描述
HRP兔单克隆抗体[EPR5367-8] to Kappa light chain -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-P, WB, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Kappa light chain.
Database link: P01834 -
阳性对照
- WB: Human tonsil and plasma tissue lysates. IHC-P: normal human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5367-8 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab202549于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
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WB |
1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa).
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ELISA |
Use a concentration of 0.01 - 10 µg/ml.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
WB
1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa). |
ELISA
Use a concentration of 0.01 - 10 µg/ml. |
靶标
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相关性
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
细胞定位
Cytoplasmic and Secreted -
数据库链接
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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别名
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
图片
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Ab202549 was tested using a sandwich ELISA approach. The wells were coated with ab195576 at 2µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Native Human Kappa Light Chain (Biorad) was then added starting at 40 µg/ml and plasma/serum at 1:200 and gradually diluted 1:4, 50µl/well for 2h. Ab202549 was then added at 1:10,000 dilution, 50µl/well for 2h.
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All lanes : HRP Anti-Kappa light chain antibody [EPR5367-8] (ab202549) at 1/5000 dilution
Lane 1 : Tonsil (Human) Whole Cell Lysate - adult normal tissue
Lane 2 : Human Plasma Total Protein Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 4 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202549 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of human kappa light chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab202549, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining corresponds to B cells expressing IgG commonly found in lymphoid follicles of human tonsil.
The inset background control image is taken from an identical assay without added antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Negative IHC image of human kappa light chain staining in a section of formalin-fixed paraffin-embedded normal human cerebral cortex*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab202549, at 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Staining corresponds to immunoglobulins found in serum. As expected, staining diminishes further into the brain tissue due to the action of the blood-brain barrier.
The inset background control image is taken from an identical assay without added antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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