Anti-KPNA2抗体(ab84440)
Key features and details
- Rabbit polyclonal to KPNA2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-KPNA2抗体
参阅全部 KPNA2 一抗 -
描述
兔多克隆抗体to KPNA2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Orangutan -
免疫原
Synthetic peptide corresponding to a region within C terminal amino acids 475-529 (ALQNHENESV YKASLSLIEK YFSVEEEEDQ NVVPETTSEG YTFQVQDGAP GTFNF) of human KPNA2 (NP_002257.1).
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常规说明
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84440于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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说明 |
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IHC-P
1/100 - 1/500. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
靶标
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功能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
组织特异性
Expressed ubiquitously. -
序列相似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
结构域
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3838 Human
- Entrez Gene: 16647 Mouse
- Omim: 600685 Human
- SwissProt: P52292 Human
- SwissProt: P52293 Mouse
- Unigene: 594238 Human
- Unigene: 12508 Mouse
- Unigene: 391577 Mouse
see all -
别名
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
see all
图片
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Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human metastatic lymph node tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded human bladder carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse squamous cell carcinoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of KPNA2 in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84440.
Detection: DAB staining. -
Immunohistochemical analysis of Human ovarian germ cell tumour, staining KPNA2 with ab84440.
Antigen retrieval was performed by submerging the sample in citrate buffer (pH 6) and microwaving. The sections were blocked with 1% BSA before incubating with primary antibody (1/400). Staining was detected using DAB.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab84440 被引用在 26 文献中.
- Miyamoto Y et al. SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. Commun Biol 5:483 (2022). PubMed: 35590097
- Dong L et al. MiR-101-3p targets KPNA2 to inhibit the progression of lung squamous cell carcinoma cell lines. Histol Histopathol N/A:18573 (2022). PubMed: 36583484
- Zhou KX et al. Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC. J Immunol Res 2021:6694392 (2021). PubMed: 33728352
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Fan X et al. Silencing of KPNA2 inhibits high glucose-induced podocyte injury via inactivation of mTORC1/p70S6K signaling pathway. Biochem Biophys Res Commun 521:1017-1023 (2020). PubMed: 31727365