Anti-DBC-1抗体(ab70242)
Key features and details
- Rabbit polyclonal to DBC-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-DBC-1抗体
参阅全部 DBC-1 一抗 -
描述
兔多克隆抗体to DBC-1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human DBC-1 aa 875-923 (C terminal).
Database link: Q8N163 -
阳性对照
- Nuclear extract from HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
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IP |
Use at 1-10 µg/mg of lysate.
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说明 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). |
IP
Use at 1-10 µg/mg of lysate. |
靶标
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功能
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. -
组织特异性
Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 464047 Chimpanzee
- Entrez Gene: 507114 Cow
- Entrez Gene: 486122 Dog
- Entrez Gene: 101135799 Gorilla
- Entrez Gene: 57805 Human
- Entrez Gene: 100173017 Orangutan
- Entrez Gene: 100155718 Pig
- Entrez Gene: 716288 Rhesus monkey
see all -
别名
- CCAR2 antibody
- Cell cycle and apoptosis regulator 2 antibody
- Cell division cycle and apoptosis regulator protein 2 antibody
see all
图片
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All lanes : Anti-DBC-1 antibody (ab70242) at 1 µg/ml
Lane 1 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : Renca (mouse renal cell carcinoma cell line) whole cell lysate
Lane 3 : 4T1 (mouse mammary gland tumor cell line) whole cell lysate
Lane 4 : CT26 (mouse colon carcinoma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 103 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DBC-1 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.
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Lanes 1-2 : Anti-DBC-1 antibody (ab70242) at 1/1000 dilution
Lanes 3-4 : Anti-DBC-1 antibody (ab70242) at 1/10000 dilution
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds -
Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab70242 被引用在 1 文献中.
- Raynes R et al. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response. PLoS One 8:e54364 (2013). WB ; Human . PubMed: 23349863