Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, HEK293, Jurkat, HL-60)
Loading amount
20 µg
Specification
HeLa, HEK293, Jurkat, HL-60
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk, PBS-Tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
supersignal west dura
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 180 kDa Non-specific: 40, 70, 100, 120 kDa
Positive control
HeLa
Additional data
Additional Notes
Lane order 1-4: HeLa, HEK293, Jurkat, HL-60
This antibody detects a weak species of the predicted mobility (180 kDa) in the HeLa positive control lysate and also in Jurkat lysate. Numerous species were observed at lower Mw's. Whether these are cross-reactive species, degradation products or alternatively spliced products is not clear. Of note, other commercial antibodies to this target also detect numerous low molecular weight species.
This antibody detects a weak species of the predicted mobility (180 kDa) in the HeLa positive control lysate and also in Jurkat lysate. Numerous species were observed at lower Mw's. Whether these are cross-reactive species, degradation products or alternatively spliced products is not clear. Of note, other commercial antibodies to this target also detect numerous low molecular weight species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Feb 22 2011