Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
100 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Chemicon Int. Donkey anti-Goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Millipore Immobilon Western Chemilumines
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: >160, ~90 kDa
Additional data
Additional Notes
100ug of Hela whole cell extracts were run on a 6%SDS-PAGE gel and bloted onto PVDF microporous membrane (Millipore ImmobilonP ). The membrane was blocked in 10% milk/ TBST and incubated with ab5199 (0.5 ug/ml) in 5% milk/ TBST. Following washes, the membrane was incubated with HRP-conjugated donkey anti-goat secondary antibody (chemicon international) diluted to 0.2 ug/ml in 5% milk/ TBST
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提交于 Jul 26 2006