Anti-KCNT1/SLACK抗体[N3/26] (ab94578)
Key features and details
- Mouse monoclonal [N3/26] to KCNT1/SLACK
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-KCNT1/SLACK抗体[N3/26]
参阅全部 KCNT1/SLACK 一抗 -
描述
小鼠单克隆抗体[N3/26] to KCNT1/SLACK -
宿主
Mouse -
特异性
Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.
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经测试应用
适用于: ICC/IF, IHC-Fr, Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment within Rat KCNT1/SLACK aa 1150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Z258 -
阳性对照
- Flow Cyt: SH-SY5Y cells. WB: Rat brain lysate.
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常规说明
The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N3/26 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94578于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000. Predicted molecular weight: 137 kDa.
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IHC-P |
1/1000.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Predicted molecular weight: 137 kDa. |
IHC-P
1/1000. |
靶标
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功能
Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). -
组织特异性
Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. -
序列相似性
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
Contains 1 RCK N-terminal domain. -
翻译后修饰
Phosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 57582 Human
- Entrez Gene: 227632 Mouse
- Entrez Gene: 60444 Rat
- Omim: 608167 Human
- SwissProt: Q5JUK3 Human
- SwissProt: Q6ZPR4 Mouse
- SwissProt: Q9Z258 Rat
- Unigene: 104950 Human
see all -
别名
- bA100C15.2 antibody
- EIEE14 antibody
- ENFL5 antibody
see all
图片
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Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-conjugated sheet anti-mouse IgG
Predicted band size: 137 kDaMembrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.
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Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab94578 被引用在 2 文献中.
- Li Y et al. A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats. Neurosci Bull 37:669-683 (2021). PubMed: 33779892
- Shore AN et al. Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy. Cell Rep 33:108303 (2020). PubMed: 33113364