Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Treatment
Dox 24 h
Specification
HEK293
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-Tween
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-mouse 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680RD
Detection
Detection method
IR
Negative control
No Dox induction
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 137 kDa Non-specific: 100 kDa
Positive control
Dox induction
Additional data
Additional Notes
Lysis buffer: RIPA + protease inhibitor + phosphatase inhibitor.
WB membrane: Nitrocellulose.
Li-Cor Odyssey scan intensity 5.0.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 03 2017