Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample
Mouse Cell (NIH3T3 TRANSFECTED KCNQ4 CELL)
Specification
NIH3T3 TRANSFECTED KCNQ4 CELL
Permeabilization
Yes - triton X100 0.5%
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ALEXA FLUOR 594GOAT ANTI MOUSE IGG (H+L9 INVITROGE
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 31 2014