Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iPSC-derived neurons)
Permeabilization
Yes - Triton
Specification
iPSC-derived neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS,1 % goat serum
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Additional data
Additional Notes
yellow colour: MAP2
red:Kv7.2
blue:DAPI
There is a streak of Kv7.2 positivity lacking MAP2 positivity, assuming axon initial segment.
The human iPSC cells were differentiated via embryoid body protocol enriched by dual SMAD inhibition.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Filip Rosa
Verified customer
提交于 Jan 24 2017