Application
Western blot
Sample
Pig Tissue lysate - whole (Right Coronary Artery & Lung)
Loading amount
20 µg
Specification
Right Coronary Artery & Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% Tris Ready Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/400
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Solution (5% Milk in TBS-Tween)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Anti Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~60 kDa Non-specific: ~140 kDa
Positive control
Porcine Lung
Additional data
Additional Notes
PVDF membrane used
Lane 1- Molecular Weight Marker
Lane 2- Porcine Right Coronary Artery (RCA)
Lane 3- Porcine Lung
Lane 4- Porcine Smooth Muscle Cell Control
Lane 5- Porcine Smooth Muscle Cell treated bFGF
Lane 6- Molecular Weight Marker
Lane 7- Porcine Right Coronary Artery (RCA) + Peptide (ab87266)
Lane 3- Porcine Lung + Peptide (ab87266)
Lane 4- Porcine Smooth Muscle Cell Control + Peptide (ab87266)
Lane 5- Porcine Smooth Muscle Cell treated bFGF + Peptide (ab87266)
Lane 1- Molecular Weight Marker
Lane 2- Porcine Right Coronary Artery (RCA)
Lane 3- Porcine Lung
Lane 4- Porcine Smooth Muscle Cell Control
Lane 5- Porcine Smooth Muscle Cell treated bFGF
Lane 6- Molecular Weight Marker
Lane 7- Porcine Right Coronary Artery (RCA) + Peptide (ab87266)
Lane 3- Porcine Lung + Peptide (ab87266)
Lane 4- Porcine Smooth Muscle Cell Control + Peptide (ab87266)
Lane 5- Porcine Smooth Muscle Cell treated bFGF + Peptide (ab87266)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 02 2010