Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain (motor cortex))
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain (motor cortex)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
27 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% goat serum with 0.1% Triton in PBS
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Anti-rabbit IgG (H+L) secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I also tried using no permeabilization in the blocking and incubation buffers, which resulted in no signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Patrick Ye
Verified customer
提交于 Aug 11 2016