Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.3% Triton
Specification
Retina
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5000µg/mL · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking solution (BSA 5mg/ml+0.3%Triton
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 donkey anti-mouse IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Rocio Gonzalez
Verified customer
提交于 Mar 27 2018