Application
ChIP
Sample
Mouse Cell lysate - nuclear (LBetaT2 Gonadotrope cell line)
Negative control
LbetaT2 chromatin immunoprecipitated with ab14984 and tested with POMC promoter primers, inactive in LBetaT2 cells
LbetaT2 chromatin immunoprecipitated with unrelevant IgG and tested with Lhb promoter specific primers
Specification
LBetaT2 Gonadotrope cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1%
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 1%
Positive control
LbetaT2 chromatin immunoprecipitated with ab14984 and tested with Lhb promoter specific primers (Mouillet JF J Biol Chem. 2004 Feb 27279(9):7832-9. Epub 2003 Dec 17.)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. David L'Hote
Verified customer
提交于 Oct 30 2017