Application
Western blot
Sample
Human Cell lysate - other (huh-7 cells)
Loading amount
10000 cells
Specification
huh-7 cells
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti-rabbit IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
SuperSignal West Pico Substrate
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 65 kDa
Additional data
Abcam response
UPDATE - Recent revidence from the customer, indicates that a band at 160 kDa is also detectable in the samples tested.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 11 2008