Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Concentration
2 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: 5% Milk/TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: HRP conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
Ecl
Bands
Specific: 120 kDa Non-specific: 65, 35 kDa
Exposure
20 minute(s) and 0 second(s)
Negative control
non-transfected HEK293 cells
Positive control
Rat and mouse lysate
Additional data
Additional Notes
1. Rat brain lysate (P31)
2. Mouse brain lysate (3mnths)
3. non-transfected HEK293 cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Garry Whitehead
Verified customer
提交于 Sep 23 2013