Anti-JNK1 + JNK2抗体(ab112501)
Key features and details
- Rabbit polyclonal to JNK1 + JNK2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-JNK1 + JNK2抗体
参阅全部 JNK1 + JNK2 一抗 -
描述
兔多克隆抗体to JNK1 + JNK2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human JNK1 + JNK2 (internal sequence).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 48 kDa.
|
|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 48 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. - Information by UniProt
-
数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 26420 Mouse
- Entrez Gene: 116554 Rat
- Entrez Gene: 50658 Rat
- Omim: 601158 Human
- Omim: 602896 Human
see all -
别名
- c jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 1 antibody
- cJun N terminal kinase 1 antibody
see all
图片
-
All lanes : Anti-JNK1 + JNK2 antibody (ab112501)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : MM231 whole cell lysate
Lane 7 : 6T-CEM whole cell lysate
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
ab112501 staining JNK1+JNK2 in Human intestinal cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
-
ab112501 staining JNK1+JNK2 in Rat intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (31)
ab112501 被引用在 31 文献中.
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Zhou Q et al. Inhibition of AEBP1 predisposes cisplatin-resistant oral cancer cells to ferroptosis. BMC Oral Health 22:478 (2022). PubMed: 36352396
- Wang Y et al. HPV16 E6E7 up-regulates KIF2A expression by activating JNK/c-Jun signal, is beneficial to migration and invasion of cervical cancer cells. Open Med (Wars) 17:1780-1787 (2022). PubMed: 36447525
- Chang C et al. Regulatory role of the TLR4/JNK signaling pathway in sepsis-induced myocardial dysfunction. Mol Med Rep 23:N/A (2021). PubMed: 33760172
- Qu Y et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182