重组PE Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)抗体[EPR5693] (ab208843)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5693] to JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)抗体[EPR5693]
参阅全部 JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) 一抗 -
描述
PE兔单克隆抗体[EPR5693] to JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
ab208843 will detect JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221). -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5693 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956)
- Alexa Fluor® 488 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab201862)
- Alexa Fluor® 647 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab201864)
- Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] - BSA and Azide free (ab219584)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208843于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF |
说明 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF |
靶标
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细胞定位
Cytoplasmic, Mitochondrial, Nuclear and Plasma membrane -
数据库链接
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 5602 Human
- Entrez Gene: 26414 Mouse
- Entrez Gene: 26419 Mouse
- Entrez Gene: 26420 Mouse
- Entrez Gene: 116554 Rat
- Entrez Gene: 25272 Rat
see all -
别名
- JNK 46 antibody
- JNK 55 antibody
- MAPK10 antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab208843 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 at 22°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208843, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow-Green laser (561nm) and 586/15 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab208843 被引用在 1 文献中.
- Hallumi E et al. Itk Promotes the Integration of TCR and CD28 Costimulation through Its Direct Substrates SLP-76 and Gads. J Immunol 206:2322-2337 (2021). PubMed: 33931484