Anti-JM4/PRAF2抗体(ab53113)
Key features and details
- Rabbit polyclonal to JM4/PRAF2
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-JM4/PRAF2抗体
参阅全部 JM4/PRAF2 一抗 -
描述
兔多克隆抗体to JM4/PRAF2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ELISA, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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说明 |
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IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
WB
1/300 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
靶标
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功能
May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis. -
组织特异性
Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles. -
序列相似性
Belongs to the PRA1 family. -
细胞定位
Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 11230 Human
- Entrez Gene: 54637 Mouse
- Entrez Gene: 367743 Rat
- SwissProt: O60831 Human
- SwissProt: Q9JIG8 Mouse
- Unigene: 29595 Human
- Unigene: 28850 Mouse
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别名
- Jena Muenchen 4 antibody
- JM 4 antibody
- PRA1 domain family member 2 antibody
see all
图片
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All lanes : Anti-JM4/PRAF2 antibody (ab53113) at 1/300 dilution
Lane 1 : Mouse spleen whole tissue lysate
Lane 2 : Mouse braint whole tissue lysate
Lane 3 : Mouse heart whole tissue lysate
Lane 4 : Mouse kidney whole tissue lysate
Lane 5 : Mouse liver whole tissue lysate
Lane 6 : Mouse embryonic fibroblasts lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible non-specific binding)
Exposure time: 20 seconds
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All lanes : Anti-JM4/PRAF2 antibody (ab53113) at 1/300 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunising peptide
Predicted band size: 19 kDa
Observed band size: 19 kDa -
ab53113 at 1/50 dilution staining JM4/PRAF2 in human brain tissue by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab53113 被引用在 2 文献中.
- He W et al. Mutual regulation of JAG2 and PRAF2 promotes migration and invasion of colorectal cancer cells uncoupled from epithelial-mesenchymal transition. Cancer Cell Int 19:160 (2019). PubMed: 31198409
- Chiu HC et al. High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells. PLoS One 9:e93305 (2014). WB ; Human . PubMed: 24671231