Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
60 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Concentration
2 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: faint 150kd kDa Non-specific: 40 kDa
Additional data
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked in your hands. Rabbit is not a validated species for this antibody and your data suggest it may not be suitable for this species under the conditions in which you used. Many thanks for your feedback on this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 15 2012