Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/30000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Yes, I had to use such a high dilution factor!
I am disappointed to find that I still cannot obtain clean, specific results.
There is positivity in occassional epidermal basal cells ( red arrowheads) and also positivity in what appear to be elastic fibres, in the dermis ( not indicated).
Please compare this image with the one I obtained using ab53112, on a sequential section.
I note that another abreview shows clear hair cuticle immunolabelling I am not sure that this is specific immunolabelling.
I am disappointed to find that I still cannot obtain clean, specific results.
There is positivity in occassional epidermal basal cells ( red arrowheads) and also positivity in what appear to be elastic fibres, in the dermis ( not indicated).
Please compare this image with the one I obtained using ab53112, on a sequential section.
I note that another abreview shows clear hair cuticle immunolabelling I am not sure that this is specific immunolabelling.
Abcam response
Thank you for submitting an Abreview. ab68 apparently does not seem to work well in this experimental procedure and specie, under these conditions. Thank you for your time, this information will surely be helpful for customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Mar 19 2012