Anti-IL-34抗体[1D12] (ab101443)
Key features and details
- Mouse monoclonal [1D12] to IL-34
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IL-34抗体[1D12]
参阅全部 IL-34 一抗 -
描述
小鼠单克隆抗体[1D12] to IL-34 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human IL-34 aa 21-242.
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阳性对照
- IL-34-hIgGFc transfected HEK293 cell lysate; U251 cells; K562 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
1D12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab101443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 27 kDa.
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 27 kDa. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R. -
组织特异性
Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. -
序列相似性
Belongs to the IL-34 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 146433 Human
- Omim: 612081 Human
- SwissProt: Q6ZMJ4 Human
- Unigene: 461214 Human
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别名
- C16orf77 antibody
- Chromosome 16 open reading frame 77 antibody
- FPT025 antibody
see all
图片
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Anti-IL-34 antibody [1D12] (ab101443) at 1/500 dilution + IL-34-hIgGFc transfected HEK293 cell lysate
Predicted band size: 27 kDa -
ab101443, at 1/200 dilution, staining IL-34 in U251 cells by Immunofluorescence analysis (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ab101443 staining IL-34 in K562 cells (green) at 1/200 by Flow cytometry. Negative control (purple).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab101443 被引用在 10 文献中.
- Arnoletti JP et al. Pancreatic Ductal Adenocarcinoma (PDAC) circulating tumor cells influence myeloid cell differentiation to support their survival and immunoresistance in portal vein circulation. PLoS One 17:e0265725 (2022). PubMed: 35316296
- Muliaditan T et al. Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors. Cell Rep Med 2:100457 (2021). PubMed: 35028604
- Lin K et al. Autocrine Production of Interleukin-34 Promotes the Development of Endometriosis through CSF1R/JAK3/STAT6 signaling. Sci Rep 9:16781 (2019). PubMed: 31727934
- Noyori O et al. Expression of IL-34 correlates with macrophage infiltration and prognosis of diffuse large B-cell lymphoma. Clin Transl Immunology 8:e1074 (2019). PubMed: 31417675
- Baghdadi M et al. High co-expression of IL-34 and M-CSF correlates with tumor progression and poor survival in lung cancers. Sci Rep 8:418 (2018). PubMed: 29323162
- Walker DG et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). PubMed: 28848420
- Baghdadi M et al. Chemotherapy-Induced IL34 Enhances Immunosuppression by Tumor-Associated Macrophages and Mediates Survival of Chemoresistant Lung Cancer Cells. Cancer Res 76:6030-6042 (2016). PubMed: 27550451
- Shoji H et al. Interleukin-34 as a fibroblast-derived marker of liver fibrosis in patients with non-alcoholic fatty liver disease. Sci Rep 6:28814 (2016). IHC-Fr ; Human . PubMed: 27363523
- Wang Y et al. IL-34 is a tissue-restricted ligand of CSF1R required for the development of Langerhans cells and microglia. Nat Immunol 13:753-60 (2012). PubMed: 22729249
- Greter M et al. Stroma-derived interleukin-34 controls the development and maintenance of langerhans cells and the maintenance of microglia. Immunity 37:1050-1060 (2012). PubMed: 23177320