Application
Western blot
Sample
Mouse Serum (Mouse Serum 0.3 ul)
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Loading amount
3 µg
Specification
Mouse Serum 0.3 ul
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/2500
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG (H + L), 0.5mg
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 17 kDa Non-specific: 31 and 76 kDa
Additional data
Additional Notes
This antibody is clearly not suitable for WB in mouse. Picture shows a series of non-specific bands.
Abcam response
Thank you for sharing this data with us. ab9657 has not previously been tested in mouse. The homology between the immunogen peptide and the mouse protein is 30% and therefore the antibody is unlikely to cross-react.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 11 2014