Anti-Interferon alpha 1抗体[F18] (ab7373)
Key features and details
- Rat monoclonal [F18] to Interferon alpha 1
- Suitable for: Neutralising, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-Interferon alpha 1抗体[F18]
参阅全部 Interferon alpha 1 一抗 -
描述
大鼠单克隆抗体[F18] to Interferon alpha 1 -
宿主
Rat -
特异性
This monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon 1. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma. -
经测试应用
适用于: Neutralising, Flow Cyt, Inhibition Assaymore details -
种属反应性
与反应: Mouse
不与反应: Human -
阳性对照
- Plasmacytoid dendritic cells.
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常规说明
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
0.2 µm filtered antibody solution. -
Primary antibody说明
With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies. -
克隆
单克隆 -
克隆编号
F18 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
Neutralising |
Use at an assay dependent concentration.
|
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 21172865
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
Inhibition Assay |
Use at an assay dependent concentration.
Useful for inhiiton in biological assays and for affinity pruification. |
|
AP |
Use at an assay dependent concentration.
|
说明 |
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Neutralising
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21172865 ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Inhibition Assay
Use at an assay dependent concentration. Useful for inhiiton in biological assays and for affinity pruification. |
AP
Use at an assay dependent concentration. |
靶标
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功能
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 15962 Mouse
- Entrez Gene: 230396 Mouse
- SwissProt: P01572 Mouse
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别名
- IFL antibody
- IFN alpha antibody
- IFN alpha 1/13 antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab7373 被引用在 7 文献中.
- Tian J et al. 5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING. EMBO J 40:e106065 (2021). PubMed: 33615517
- Büssow K et al. ER intrabody-mediated inhibition of interferon a secretion by mouse macrophages and dendritic cells. PLoS One 14:e0215062 (2019). PubMed: 30990863
- Bi E et al. E-cadherin expression on multiple myeloma cells activates tumor-promoting properties in plasmacytoid DCs. J Clin Invest 128:4821-4831 (2018). PubMed: 30277474
- Biswas I et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). PubMed: 26350442
- Castellaneta A et al. Plasmacytoid dendritic cell-derived IFN-a promotes murine liver ischemia/reperfusion injury by induction of hepatocyte IRF-1. Hepatology 60:267-77 (2014). PubMed: 24493010
- Björck P et al. Plasmacytoid dendritic cell dichotomy: identification of IFN-a producing cells as a phenotypically and functionally distinct subset. J Immunol 186:1477-85 (2011). Flow Cyt ; Mouse . PubMed: 21172865
- Joshi VD et al. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J Interferon Cytokine Res 26:739-47 (2006). PubMed: 17032168