Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
25 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat ant-rabbit IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Licor
Exposure
20 second(s)
Bands
Specific: 55 kDa
Positive control
platelet
Additional data
Additional Notes
We used this Abcam antibody to probe for ILK in human platelets and Chinese hamster ovary cells (CHO). The antibody gave a clean band at approximately 55 kDa as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 04 2012