Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (gastric epithelial cell)
Specification
gastric epithelial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
Other product details
Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 28°C · Diluent: DAKO antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Sigma
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/80
Additional data
Additional Notes
Nuclei were counterstained with propidium iodide.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. J Chai
Verified customer
提交于 Apr 14 2012