Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Other product details
Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Cy3-AffiniPure F(ab')2 Fragment Goat Anti-Mouse Ig
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Monoclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
doesn't work
Discount code: 52185
Discount code: 52185
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in immunocytochemistry using human sample. This antibody has not been tested with this application before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 05 2011