Application
Flow Cytometry
Sample
Human Cell (melanoma cell line)
Specification
melanoma cell line
Preparation
Cell harvesting/tissue preparation method: cell line - trypsinized cells, treated with STI and spun out trypsin, kept on ice during experiment duration
Sample buffer: 2% FCS/0.1%NaAzide/PBS
Sample buffer: 2% FCS/0.1%NaAzide/PBS
Fixation
none
Permeabilization
No
Gating Strategy
FSC and SSC for healthy cell population, excluded PI positive cells
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% FCS/0.1%NaAzide/PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse *highly conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
this worked much better than an Integrin alpha V (SMA-2) antibody from another company which did not detect any signal by FACS.
Highly recommend this antibody- it worked beautifully for IF as well.
Highly recommend this antibody- it worked beautifully for IF as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 15 2009