重组Anti-Integrin alpha 2抗体[EPR5789] (ab109432)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5789] to Integrin alpha 2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Integrin alpha 2抗体[EPR5789]
参阅全部 Integrin alpha 2 一抗 -
描述
兔单克隆抗体[EPR5789] to Integrin alpha 2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HAP1, 293T, T47-D, and A431 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5789 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 129 kDa. |
靶标
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功能
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3673 Human
- Omim: 192974 Human
- SwissProt: P17301 Human
- Unigene: 482077 Human
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别名
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
see all
图片
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All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ITGA2 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 129 kDaLanes 1-4: Merged signal (red and green). Green - ab109432 observed at 129 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109432 Anti-Integrin alpha 2 antibody [EPR5789] was shown to specifically react with Integrin alpha 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264819 (knockout cell lysate ab257262) was used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Integrin alpha 2 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : T47D cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 129 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109432 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109432 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab109432 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-Integrin alpha 2 antibody [EPR5789] (ab109432) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 129 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109432 被引用在 1 文献中.
- Guo F et al. Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair? PLoS One 6:e27097 (2011). WB ; Human . PubMed: 22073262