Western blot abreview for Anti-Insulin Receptor (phospho Y972) antibody

Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Treatment 5gy Irradiation
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution 1/1000
Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 100ug/ml BSA

Secondary antibody

Name Non-Abcam antibody was used: Anti-rabbit IgG, HRP linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/2000

Detection

Detection method ECL
Exposure 15 minute(s) and 0 second(s)
Bands Specific: 110 kDa

Additional data

Additional Notes all samples - tumor whole lysates.
=> 2nd, 4th lanes-under 5gy irradiation.
Username

Ms. Seontae Kim

Verified customer

提交于 Jan 14 2013