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No signal detected in western blots of insulin-treated controls or experimental samples.
Asked on Oct 06 2011
Thank you for contacting us about the issue you are having with ab5678. I noticed after we spoke that the datasheet for the antibody I proposed sending, the rabbit monoclonal anti-Insulin Receptor (phospho Y1185), has a note stating that it will not react with mouse samples. I went back to the list we were studying and found another that may be a better choice: Insulin Receptor (phospho Y1158) antibody (ab78355) Click here (or use the following: http://www.abcam.com/index.html?datasheet=78355). The antibody has not been tested against mouse samples but is raised against a sequence conserved in mouse. I will offer our guarantee for this antibody, in case it does not work for you. We usually only offer a guarantee for tested species and applications, but I am fairly confident this will be able to detect the phosphorylation in mouse samples. Please let me know if you would like to try this, ab78355, instead of the rabbit monoclonal, which we will not guarantee, given the negative data for mouse. The antibody is in stock and available for overnight delivery.
Abcam Scientific Support
回复于 Oct 06 2011