Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Denaturing (10)
Sample
Mouse Tissue lysate - other (Hep G2)
Specification
Hep G2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: promega anti-mouse Ig HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 110 kDa Non-specific: 80 kDa
Exposure
15 second(s)
Negative control
empty WGA beads
Positive control
cell lysis
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 27 2014