重组Anti-Inhibin alpha抗体[EPY2782] - BSA and Azide free (ab226844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPY2782] to Inhibin alpha - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Inhibin alpha抗体[EPY2782] - BSA and Azide free
参阅全部 Inhibin alpha 一抗 -
描述
兔单克隆抗体[EPY2782] to Inhibin alpha - BSA and Azide free -
宿主
Rabbit -
特异性
Note: 20/23 kDa bands consist of the mature alpha chain. 50/53 kDa bands are the entire proprotein.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human adrenal gland and placenta lysate; HeLa and LNCaP cell lysates.
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常规说明
ab226844 is the carrier-free version of ab81322.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPY2782 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
靶标
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功能
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
组织特异性
Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3623 Human
- Entrez Gene: 16322 Mouse
- Entrez Gene: 24504 Rat
- Omim: 147380 Human
- SwissProt: P05111 Human
- SwissProt: Q04997 Mouse
- SwissProt: P17490 Rat
- Unigene: 407506 Human
see all -
别名
- A inhibin subunit antibody
- A inhibin subunit precursor antibody
- AW55578 antibody
see all
图片
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All lanes : Anti-Inhibin alpha antibody [EPY2782] (ab81322) at 1/2000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Human placenta lysate
Lane 4 : LNCaP (Human prostate cancer cell line) cell lysate
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 40 kDaNote: the 50 kDa band corresponds to the entire proprotein.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81322).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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