重组Anti-Indoleamine 2,3-dioxygenase抗体[EPR1230Y] - Low endotoxin,Azide free (ab224262)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase - Low endotoxin, Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
概述
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产品名称
Anti-Indoleamine 2,3-dioxygenase抗体[EPR1230Y] - Low endotoxin,Azide free
参阅全部 Indoleamine 2, 3-dioxygenase 一抗 -
描述
兔单克隆抗体[EPR1230Y] to Indoleamine 2,3-dioxygenase - Low endotoxin,Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type A549 Treated IFN gamma, SK-OV-3 and HeLa treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate.
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常规说明
ab224262 is the carrier-free version of ab76157.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1230Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
靶标
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功能
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
通路
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
序列相似性
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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数据库链接
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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别名
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
图片
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All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1 : Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 2 : Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 3 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 4 : IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 5 : SK-OV-3 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab76157).
L
Lanes 1 - 6: Merged signal (red and green). Green - ab76157 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab76157 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with loss of signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab76157 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Lane 1 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
Lane 2 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaThe expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (2)
ab224262 被引用在 2 文献中.
- Yamazaki T et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . PubMed: 24855914
- De Ravin SS et al. Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide and is fully maintained in chronic granulomatous disease. Blood 116:1755-60 (2010). PubMed: 20511543