重组Anti-IgA抗体[EPR5367-76] (ab124716)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5367-76] to IgA
- Suitable for: WB, IP, ELISA, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IgA抗体[EPR5367-76]
参阅全部 IgA 一抗 -
描述
兔单克隆抗体[EPR5367-76] to IgA -
宿主
Rabbit -
特异性
This antibody reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.
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经测试应用
适用于: WB, IP, ELISA, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human IgA.
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阳性对照
- Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5367-76 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124716于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
1/10 - 1/100.
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ELISA |
1/250 - 1/4000.
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
1/10 - 1/100. |
ELISA
1/250 - 1/4000. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 3494 Human
- Entrez Gene: 5284 Human
- Omim: 146900 Human
- Omim: 147000 Human
- Omim: 173880 Human
- SwissProt: P01876 Human
- SwissProt: P01877 Human
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别名
- Hepatocellular carcinoma-associated protein TB6 antibody
- Ig alpha 1 chain C region antibody
- Ig alpha 2 chain C region antibody
see all
图片
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Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma
All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 0.18 µg/ml (ab124716 at 1:1000 dilution (0.18µg/ml))
Lane 1 : Human plasma with NFDM/TBST
Lane 2 : Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma with NFDM/TBST
Blocking peptides at 5 % per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab124716 被引用在 5 文献中.
- Biswas S et al. IgA transcytosis and antigen recognition govern ovarian cancer immunity. Nature N/A:N/A (2021). PubMed: 33536615
- Verathamjamras C et al. Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer. Glycoconj J 38:55-65 (2021). PubMed: 33608772
- Liu H et al. Small airway immunoglobulin A profile in emphysema-predominant chronic obstructive pulmonary disease. Chin Med J (Engl) 133:1915-1921 (2020). PubMed: 32826454
- Shulzhenko N et al. CVID enteropathy is characterized by exceeding low mucosal IgA levels and interferon-driven inflammation possibly related to the presence of a pathobiont. Clin Immunol 197:139-153 (2018). PubMed: 30240602
- Fahrbach KM et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . PubMed: 24098437