Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast, prostate)
Specification
breast, prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Other product details
Dilution
1/40
Incubation time
15 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducing agents
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse/rabbit IgG containing 10% animal serum
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Staining is confined to endothelial cells and fibroblasts within stroma of breast and prostate cancer.
Various retrieval protocols (proteinase K enzyme, citrate, EDTA,no ptt) citrate at 100C-steamer for 20 minutes gave best results with polymer detection (Leica Microsystems Refine) with DAB and hematoxylin counterstain
Various retrieval protocols (proteinase K enzyme, citrate, EDTA,no ptt) citrate at 100C-steamer for 20 minutes gave best results with polymer detection (Leica Microsystems Refine) with DAB and hematoxylin counterstain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 02 2009