Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
A region within synthetic peptide: MSLLCRNKGC GQHFDPNTNL PDSCCHHPGV PIFHDALKGW SCCRKRTVDF, corresponding to N terminal amino acids 1-50 of Human ITGB1BP2
- Fetal heart cell lysate. Human muscle.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/312500.
||Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
ITGB1BP2 is a muscle-specific protein that interacts with the Integrin 1 cytoplasmic domain, in the hypertrophic response to mechanical overload.
- CHORDC3 antibody
- Integrin beta 1 binding protein (melusin) 2 antibody
- integrin beta 1 binding protein 2 antibody
Western blot - Anti-ITGB1BP2 antibody (ab62300)
ITGB1BP2 antibody (ab62300) at 2.5µg/ml (in 5% skim milk / PBS buffer) + Fetal heart cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
36 kDaObserved band size:
39 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB1BP2 antibody (ab62300)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling ITGB1BP2 with ab62300 at 4-8µg/ml. Arrows indicate positively stained skeletal muscle cells. Magnification: 400X.
has not yet been referenced specifically in any publications.
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