Anti-IRGM抗体(ab69494)
Key features and details
- Rabbit polyclonal to IRGM
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IRGM抗体
参阅全部 IRGM 一抗 -
描述
兔多克隆抗体to IRGM -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A 17 amino acid peptide near the amino terminus of human IRGM.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 21102437
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.
Please refer to databases and the literature for the expected molecular weights of species orthologues. |
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 21102437 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 20 kDa. Please refer to databases and the literature for the expected molecular weights of species orthologues. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. -
组织特异性
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes. -
疾病相关
Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. -
序列相似性
Belongs to the interferon-inducible GTPase family. -
细胞定位
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 345611 Human
- SwissProt: A1A4Y4 Human
- Unigene: 519680 Human
- Unigene: 652148 Human
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别名
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
see all
图片
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Paraffin-embedded human thymus tissue stained for IRGM using ab69494 at 5 µg/ml in immunohistochemical analysis.
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IHC image of ab69494 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69494, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab69494 被引用在 11 文献中.
- Yang L et al. Lactobacillusjohnsonii L531 Protects against Salmonella Infantis-Induced Intestinal Damage by Regulating the NOD Activation, Endoplasmic Reticulum Stress, and Autophagy. Int J Mol Sci 23:N/A (2022). PubMed: 36142312
- Nath P et al. Inhibition of IRGM establishes a robust antiviral immune state to restrict pathogenic viruses. EMBO Rep 22:e52948 (2021). PubMed: 34467632
- Jena KK et al. Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response. EMBO Rep 21:e50051 (2020). PubMed: 32715615
- Kumar S et al. Mammalian Atg8 proteins and the autophagy factor IRGM control mTOR and TFEB at a regulatory node critical for responses to pathogens. Nat Cell Biol 22:973-985 (2020). PubMed: 32753672
- Mehto S et al. The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Mol Cell 73:429-445.e7 (2019). PubMed: 30612879