Application
Flow Cytometry
Sample
Human Cell (primary human macrophages and CD4+ T cells)
Permeabilization
Yes - BD Cytofix/cytoperm kit
Gating Strategy
all live cells
Specification
primary human macrophages and CD4+ T cells
Preparation
Cell harvesting/tissue preparation method: Monocyte-derived macrophages were prepared from human PBMCs CD4+ T cells were separated from human PBMCs
Sample buffer: 0.5%BSA/PBS
Sample buffer: 0.5%BSA/PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 21°C · Diluent: BD perm/wash buffer
Dilution
1/200
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: donkey anti-rabbit AF647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
The antibody was used for Imaging cytometry (a combination of flowcyotometry and microscopy) with an ImageStream instrument (Millipore). It allowed quantification of IRF3 nuclear translocation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Radiana Trifonova
Verified customer
提交于 May 27 2016