Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mesenteric artery)
Permeabilization
No
Specification
mesenteric artery
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Acetone
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS BSA3%
Dilution
1/500
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Jackson immunoresearch 711-165-152
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
P-IRE1 staining with Cy3-coupled secondary antibody (red).
Nuclear staining with DAPI (blue).
Autofluorescence of the extracellular matrix appears in green.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 06 2015