Anti-IRE1 (phospho S724)抗体(ab48187)
Key features and details
- Rabbit polyclonal to IRE1 (phospho S724)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IRE1 (phospho S724)抗体
参阅全部 IRE1 一抗 -
描述
兔多克隆抗体to IRE1 (phospho S724) -
宿主
Rabbit -
经测试应用
适用于: WB, ELISA, IHC-Pmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
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阳性对照
- WB: HeLa, Min6, transfected COS-7 cells. IHC-P: Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (14) |
1/1000 - 1/2000. Predicted molecular weight: 110 kDa.
Block with 3-5% BSA. |
ELISA |
1/100 - 1/2000.
|
|
IHC-P | (1) |
1/300.
|
说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 110 kDa. Block with 3-5% BSA. |
ELISA
1/100 - 1/2000. |
IHC-P
1/300. |
靶标
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功能
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
组织特异性
Ubiquitously expressed. High levels observed in pancreatic tissue. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Entrez Gene: 78943 Mouse
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- SwissProt: Q9EQY0 Mouse
- SwissProt: Q9Z2E3 Mouse
see all -
别名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
图片
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All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution
Lane 1 : HeLa cells 30 nM Calyculin A: AP-buffer
Lane 2 : HeLa cells 30 nM Calyculin A: Alkaline Phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDaThe blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membranes were blocked for an hour. Membrane 2 was incubated with alkaline phosphatase (AP; 100 U per mL) for one hour, whilst membrane 1 was treated with AP-buffer only, before being incubated with ab48187 (rabbit anti-IRE1 antibody diluted 1:2000) and loading control ab125247 (mouse anti-GAPDH antibody; diluted 1:10,000) for 24 hours at 4°C. Antibody binding was detected using infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1:10,000 dilutions for 1 hour at room temperature before imaging.
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Paraffin-embedded human spleen tissue stained for IRE1 using ab48187 at 1/300 dilution for 1 hour at room temperature in immunohistochemical analysis. DAB staining. Counter stained with hematoxylin.
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Serially diluted ab48187 was bound to immobilised Phospho peptide (133861) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.
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All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/1000 dilution
Lane 1 : Min6 cells untreated
Lane 2 : Min6 cells treated with glucose for 3 hours at 5 mM
Lane 3 : Min6 cells treated with glucose for 3 hours at 20 mM
Developed using the ECL technique.
Predicted band size: 110 kDa -
All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution
Lane 1 : Cell lysate prepared from COS-7 Untransfected cells
Lane 2 : Cell lysate prepared from COS-7 cells expressing wild type IRE1 alpha
Lane 3 : Cell lysate prepared from COS-7 cells expressing kinase-dead IRE1 alpha
Predicted band size: 110 kDa -
All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 2 µg/ml
Lane 1 : Untreated HeLa cells (Batch 1 ab48187)
Lane 2 : DTT-treated HeLa cells (Batch 1 ab48187)
Lane 3 : Untreated HeLa cells (Batch 2 ab48187)
Lane 4 : DTT-treated HeLa cells (Batch 2 ab48187)
Secondary
All lanes : Anti-Rabbit IgG HRP
Predicted band size: 110 kDaHeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (257)
ab48187 被引用在 257 文献中.
- Yang Y et al. Glycine represses endoplasmic reticulum stress-related apoptosis and improves intestinal barrier by activating mammalian target of rapamycin complex 1 signaling. Anim Nutr 8:1-9 (2022). PubMed: 34977370
- Weingartner M et al. Albendazole reduces hepatic inflammation and endoplasmic reticulum-stress in a mouse model of chronic Echinococcus multilocularis infection. PLoS Negl Trop Dis 16:e0009192 (2022). PubMed: 35030165
- Yilmaz DE et al. Immunosuppressive calcineurin inhibitor cyclosporine A induces proapoptotic endoplasmic reticulum stress in renal tubular cells. J Biol Chem 298:101589 (2022). PubMed: 35033536
- Andrés-Benito P et al. Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum. Int J Mol Sci 23:N/A (2022). PubMed: 35054902
- Mao N et al. Oxamate Attenuates Glycolysis and ER Stress in Silicotic Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35328434