重组Anti-IRE1 (phospho S724)抗体[EPR5253] (ab124945)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5253] to IRE1 (phospho S724)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-IRE1 (phospho S724)抗体[EPR5253]
参阅全部 IRE1 一抗 -
描述
兔单克隆抗体[EPR5253] to IRE1 (phospho S724) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human IRE1 aa 700-800 (phospho S724). The exact sequence is proprietary.
Database link: O75460 -
阳性对照
- WB: Thapsigargin treated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, DTT treated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.10 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5253 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124945于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (5) |
1/200 - 1/10000. Predicted molecular weight: 110 kDa.
|
Dot blot |
1/1000.
|
说明 |
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WB
1/200 - 1/10000. Predicted molecular weight: 110 kDa. |
Dot blot
1/1000. |
靶标
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功能
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
组织特异性
Ubiquitously expressed. High levels observed in pancreatic tissue. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- Unigene: 133982 Human
- Unigene: 592041 Human
- Unigene: 700027 Human
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别名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
图片
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All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/200 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 1 hour whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 6 hours whole cell lysates
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) treated with 10mM DTT for 1 hour whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
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Dot Blot analysis of Lane 1: IRE1 (pS724) phospho peptide and Lane 2: IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (92)
ab124945 被引用在 92 文献中.
- Chung AW et al. Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6. NPJ Breast Cancer 8:30 (2022). PubMed: 35260569
- Lee HY et al. GABA and Fermented Curcuma longa L. Extract Enriched with GABA Ameliorate Obesity through Nox4-IRE1α Sulfonation-RIDD-SIRT1 Decay Axis in High-Fat Diet-Induced Obese Mice. Nutrients 14:N/A (2022). PubMed: 35458241
- Pillai S et al. Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy. Cell Rep 39:110796 (2022). PubMed: 35545051
- Fan H et al. α-Linolenic Acid Suppresses Proliferation and Invasion in Osteosarcoma Cells via Inhibiting Fatty Acid Synthase. Molecules 27:N/A (2022). PubMed: 35566090
- Chen L et al. Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor. Elife 11:N/A (2022). PubMed: 35998104