Anti-IRAK-1 (phospho T387)抗体(ab112042)
Key features and details
- Rabbit polyclonal to IRAK-1 (phospho T387)
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-IRAK-1 (phospho T387)抗体
参阅全部 IRAK-1 一抗 -
描述
兔多克隆抗体to IRAK-1 (phospho T387) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Human IRAK-1 (phospho T387).
Database link: P51617 -
阳性对照
- NIH3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Frozen Stock (-20C). Shelf life 12 months. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab112042 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112042于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/100 - 1/500.
|
说明 |
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ICC/IF
1/100 - 1/500. |
靶标
-
功能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
组织特异性
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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数据库链接
- Entrez Gene: 16179 Mouse
- Entrez Gene: 363520 Rat
- SwissProt: Q62406 Mouse
- Unigene: 38241 Mouse
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别名
- AA48924 antibody
- Il1rak antibody
- Interleukin 1 receptor associated kinase 1 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab112042 尚未被引用在任何文献中。